Plasma 8-isoprostane concentrations in patients with age-related cataracts.

Terstappen LW. Changes in circulating carcinoma cells in patients with metastatic prostate cancer correlate with disease status. Urology 2001;58: 386 –92. vitro detection of prostate cancer circulating cells by immunocy-tochemistry, flow cytometry and RT-PCR PSA. Circulating prostate specific antigen-positive cells correlate with metastatic prostate cancer. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. al. Human immunodeficiency virus type 1 (HIV-1) antigen secretion by latently infected resting CD4ϩ T lymphocytes from HIV-1-infected individuals. Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity. Fluorospot assay to detect single T lymphocytes simultaneously producing multiple cytokines. A novel two color ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells. A novel two color ELISPOT assay. al. Spontaneous monoclonal immunoglobulin-secreting peripheral blood mononuclear cells as a marker of disease severity in multiple myeloma. in the peripheral blood of breast cancer patients. Quantification of prostate specific antigen mRNA levels in circulation after prostatic surgery and endocrine treatment by quantitative reverse transcription -polymerase chain reaction. cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Cataracts are the most common cause of blindness and visual dysfunction in the world. Cataractogenesis is a highly complex, multifactorial process. Epidemiologic studies have shown that potential risk factors include age, sex female, exposure to ultraviolet light, smoking, diabetes , and oxidative stress (1– 4). Opacification of the ocular lens may be initiated or promoted by oxidative damage, and data in the literature support an important role of oxidative damage in cataract formation (5, 6). Although animal experiments show evidence for a protective role of antioxidants (7, 8), the association between low concentrations of antioxidants and increased risk of cataracts remains controversial. Whereas some studies have demonstrated such associations, others have not (9, 10). Isoprostanes are a complex family of compounds produced from arachidonic acid. One of the isoprostanes, 8-isoprostaglandin F 2␣ (8-iso-PGF 2␣), belongs to a family of eicosanoids of nonenzymatic origin (11). 8-Iso-PGF 2␣ has been widely used as a valid marker of oxidative stress (12, 13). Several methods are currently used to quantify 8-iso-PGF 2␣ , including gas chromatography–mass spec-trometry, gas chromatography–tandem mass spectrome-try, and liquid chromatography–tandem mass spectrom-etry (14, 15), but their cost and technologic requirements limit their routine use. Recently, immunoassays have also been developed to measure 8-iso-PGF 2␣ (16, 17). Koliakos et al. (18) found that the …